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61.
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63.
Dae Hong Lee Mi Ja Seo Eun Jin Kang Cho Rong Park Chang Wook Jo In Cheon Hwang Yong Man Yu Young Nam Youn 《Journal of Asia》2011,14(1):99-105
The Asian ladybird beetle, Harmonia axyridis shows polymorphism in elytra color patterns. However, it is uncertain whether these color patterns are regulated by genetic factors. This investigation used amplified fragment length polymorphism (AFLP) analysis to determine any genetic causes of the variability of color patterns. Using four individuals of each group, AFLP analysis produced 37 polymorphic bands. Among several polymorphic bands, six AFLP markers were associated with elytra color patterns after further analysis using six additional individuals of each group. These polymorphic sites were sequenced but did not match DNA sequence data deposited in GenBank. Based on the color-associated AFLP markers, SCAR primers were designed for PCR amplification of genomic DNA. These primers (SCAR 12 and SCAR 44) were used to analyze color-associated loci and/or alleles of H. axyridis DNA. SCAR 12 primers designed from a Spectabilis type-specific fragment (AFLP 12) amplified a specific band of 530 bp in four Spectabilis individuals, but not in the insects with other color patterns. 相似文献
64.
So-Jung Choi Sung-Hyun Kim Ho Y. Kang Jinseon Lee Jong H. Bhak Insuk Sohn Sin-Ho Jung Yong Soo Choi Hong Kwan Kim Jungho Han Nam Huh Gyusang Lee Byung C. Kim Jhingook Kim 《Biochemical and biophysical research communications》2011,(1):23
We determined the somatic mutations in the mitochondrial genomes of 70 lung cancer patients by pair-wise comparative analyses of the normal- and tumor-genome sequences acquired using Affymetrix Mitochondrial Resequencing Array 2.0. The overall mutation rates in lung cancers were Approximately 100 fold higher than those in normal cells, with significant statistical correlation with smoking (p = 0.00088). Total of 532 somatic mutations were evenly distributed in 499 positions with very low overall frequency (1.07/bp), but the non-synonymous mutations causing amino acid substitution occurred more frequently (1.83/bp), particularly at two positions, 8701 and 10398 (10.5/bp) that code for ATPase6 and NADH dehydrogenase 3, respectively. Despite the randomness or even distribution of the mutations, these two mutations occurred together in 86% of the cases. The linkage between the two most frequent mutations suggests that they were selected together, possibly due to their cooperative role during cancer development. Indeed, the mutation at 10398 was shown by Canter, Pezzotti, and their colleagues in 2009, as a risk factor for breast cancer. In this study, we identified two potential biomarkers that might be functionally linked together during the development of cancer. 相似文献
65.
Nam Hee Yoo Ok Tae Kim Jung Bong Kim Sun Hee Kim Young Chang Kim Kyong Hwan Bang Dong Yun Hyun Seon Woo Cha Min Young Kim Baik Hwang 《Plant biotechnology reports》2011,5(3):283-287
In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and
centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of madecassoside
and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation
alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to
the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and
TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the
accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation. 相似文献
66.
Kyung Won Seo Su Jin Heo Yowhan Son Nam Jin Noh Sue Kyoung Lee Chun Gyeong Yoon 《Landscape and Ecological Engineering》2011,7(1):93-99
This study was conducted to examine the influences of soil-moisture conditions on soil nitrogen (N) dynamics, including in
situ soil N mineralization, N availability, and denitrification in a pure Alnus japonica forest located in Seoul, central Korea. The soil N mineralization, N availability, and denitrification were determined using
the buried bag incubation method, ion exchange resin bag method, and acetylene block method, respectively. The annual net
N mineralization rate (kg N ha−1 year−1) and annual N availability (mg N bag−1) were 40.26 and 80.65 in the relatively dry site, −5.43 and 45.39 in the moist site, and 7.09 and 39.17 in the wet site,
respectively. The annual net N mineralization rate and annual N availability in the dry site were significantly higher than
those in the moist and wet sites, whereas there was no significant difference between the moist and wet sites. The annual
mean denitrification rate (kg N ha−1 year−1) in the dry, moist, and wet sites was 2.37, 2.76, and 1.59, respectively. However, there was no significant difference among
sites due to the high spatial and temporal variations. Our results indicate that soil-moisture condition influenced the in
situ N mineralization and resin bag N availability in an A. japonica forest, and treatments of proper drainage for poorly drained sites would increase soil N mineralization and N availability
and consequently be useful to conserve and manage the A. japonica forest. 相似文献
67.
Moongeun Yoon Keun-Yong Kim In-Chul Bang Yoon Kwon Nam Dong Soo Kim 《Genes & genomics.》2011,33(3):307-312
Chinese medaka Oryzias sinensis (Teleostei: Beloniformes) is distributed in China and the western areas of Korea. It differs from the Japanese medaka O. latipes in its morphology and biogeographical distribution in Korea. In this study, we analyzed the complete nucleotide sequence of the mitochondrial genome (mitogenome) of O. sinensis and determined its genomic structure. The complete mitogenome is a circular molecule of 16,654 bp and its structural organization is conserved across diverse vertebrate taxa. On a phylogenetic tree inferred from the nucleotide matrix, partitioned into four regions (i.e., the first and second codon positions of protein-coding genes, and the ribosomal RNA and transfer RNA genes), O. sinensis clustered consistently with O. latipes specimens from China and Korea, with a relatively short branch length, but was separated from O. latipes specimen from Japan. Our findings will allow the resolution of the taxonomic problems of closely related Oryzias species, such as O. sinensis and O. latipes in a future study. 相似文献
68.
Kung Ahn Jin-Han Bae Kyu-Hwi Nam Chong-Eon Lee Kyung-Do Park Hak-Kyo Lee Byung-Wook Cho Heui-Soo Kim 《Genes & genomics.》2011,33(3):245-250
Quantitative analysis of horse gene expression profiles under diverse experimental conditions is limited by the lack of reliable reference genes for normalization of mRNA levels. Therefore, in this study, the expression of potential reference genes was compared between thoroughbred and Jeju native horse (Jeju pony). We compared the expression of nine genes by quantitative real-time RT-PCR in fourteen tissues between the two horse breeds and analyzed their stability using the geNorm and NormFinder programs. The data obtained in this study suggest that the UBB gene could serve as a reference gene in gene expression analysis of thoroughbred and Jeju native horses. 相似文献
69.
Jeffrey D Orth Tom M Conrad Jessica Na Joshua A Lerman Hojung Nam Adam M Feist Bernhard Ø Palsson 《Molecular systems biology》2011,7(1)
The initial genome‐scale reconstruction of the metabolic network of Escherichia coli K‐12 MG1655 was assembled in 2000. It has been updated and periodically released since then based on new and curated genomic and biochemical knowledge. An update has now been built, named iJO1366, which accounts for 1366 genes, 2251 metabolic reactions, and 1136 unique metabolites. iJO1366 was (1) updated in part using a new experimental screen of 1075 gene knockout strains, illuminating cases where alternative pathways and isozymes are yet to be discovered, (2) compared with its predecessor and to experimental data sets to confirm that it continues to make accurate phenotypic predictions of growth on different substrates and for gene knockout strains, and (3) mapped to the genomes of all available sequenced E. coli strains, including pathogens, leading to the identification of hundreds of unannotated genes in these organisms. Like its predecessors, the iJO1366 reconstruction is expected to be widely deployed for studying the systems biology of E. coli and for metabolic engineering applications. 相似文献
70.
Woo Yong Sung Ji Won Yu Jong Tae Hwang Hee Jin Nam Ji Ye Park Yongae Kim Jang‐Hee Cho 《Journal of peptide science》2020,26(8)
Antimicrobial peptides are class of small, positively charged peptides known for their broad‐spectrum antimicrobial activity. Antimicrobial activities for most antimicrobial peptides have largely remained elusive, particularly in the lactic acid bacteria. However, recently our investigation using LPcin‐YK3, an antimicrobial peptide from bovine milk, suggests that in vitro antimicrobial activity was reduced over 100‐fold compared with pathogenic bacteria. Additionally, for the structural study of how antimicrobial peptide undergoes its reaction at the proteolytic pathway of lactic acid bacteria based on degradation assay and propidium iodide staining, we performed molecular docking for interaction between oligopeptide‐binding protein A and LPcin‐YK3 peptide. Given that degradation related to the LPcin‐YK3 peptide in lactic acid bacteria proteolytic system, the inhibitory inactivity of LPcin‐YK3 against beneficial lactic acid bacteria strains may be one of the primary pharmacological properties of recombinant peptide discovered in bovine milk. These results provide structural and functional insights into the proteolytic mechanism and possibility as a putative substrate of oligopeptide‐binding protein A in respect of LPcin‐YK3 peptide. 相似文献